for permeabilization of cells before techniques like indirect immunofluorescence and flow cytometry
As a component of lysis buffer used for homogenization of cells
As a component of blocking buffer in immunofluorescence staining
For the preparation of cells in double fluorescence staining
Biochem/physiol Actions
Widely used non-ionic surfactant for recovery of membrane components under mild non-denaturing conditions.
General description
Triton™ X-100 is a common non-ionic surfactant and emulsifier which is often used in biochemical applications to solubilize proteins. It is considered a comparatively mild detergent, non-denaturing, and is reported in numerous references as a routinely added reagent. It is utilized for lysing cells to extract protein and cellular organelles. It can also permeabilize the living cell membrane for transfection.
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