B0300
Sigma-Aldrich

BETAINE, 5M

Linear Formula:

(CH3)3N+CH2COO

Empform:

C5H11NO2

Nacres: Betaine solution
Fw:
Fema No: 24891511
CAS Number: 107-43-7
Grouped product items
SKU Pack Size Availability Price Quantity
B0300-5VL - Available to ship on March 24, 2024
IDR 2,700,000.00
B0300-1VL - Available to ship on March 24, 2024
IDR 783,000.00
Request a Bulk Order Check Availability

Properties

Properties
applications agriculture
concentration
concentricity M
Description BETAINE, 5M
form DNase RNase none detected
format liquid
gradient analysis PCR Reagent
inchi key 1S/C5H11NO2/c1-6(23)4-5(7)8/h4H21-3H3
indiff. to chromic acid KWIUHFFTVRNATP-UHFFFAOYSA-N
MDM Code B0300-5VL
Material Number B0300
Business Unit 042
Shipping Mode AIR
MRP Type YP
Local HS Code 29239000
Legacy SIGMA
Plant 6311
Sales Org 6304
CAS Number 107-43-7

Description

Application

Betaine solution has been used as a component of the reaction mixture for:
  • loop-mediated isothermal amplification (LAMP) reactions
  • polymerase chain reaction for genomic DNA amplification
  • quantitative polymerase chain reaction (qPCR) for telomere length analysis
  • in the PCR amplification of CGG repeats in the genomic DNA
  • reverse-transcription loop-mediated isothermal amplification (RT-LAMP) to detect severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2).
  • PCR amplification of DNA-directed RNA polymerase II largest subunit (RPB2)
  • reverse transcription during library preparation using Smartseq2

General description

Betaine also called trimethylglycine or N,N,N triethylammonium acetate, is an analog of glycine with three methyl groups. It is highly compatible with polymerase chain reaction (PCR) buffer mixture. Betaine is a PCR enhancing reagent that is widely used for improving the yield and specificity of PCR products, especially during the PCR amplification of targets rich in GC content or those that form secondary structures resulting in poor yield. Betaine facilitates DNA strand separation and manages the DNA melting temperature (Tm) difference between the GC and AT pairs in DNA. It stabilizes the ds DNA by equalizing the contribution of GC- and AT-base pairs. Betaine has been broadly used to optimize multiplex and ‘long and accurate’ polymerase chain reaction (LA-PCR). The addition of 1.0-1.7 M aqueous betaine to a PCR mixture has been reported to reduce the base pair composition dependence on DNA strand melting.

Packaging

1.5mL per vial

SAFETY INFORMATION

Signal Word

RIDADRRIDADR

WGK Germany

WGK 3

Flash Point (F)

Not applicable

Flash Point (C)

Not applicable

Target Organs

Risk Statement

Supplemental Hazard Statements

DOCUMENTATION

Certificate of Analysis View Sample COA
Certificate of Origin

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