Chondroitinase AC from Flavobacterium heparinum lyophilized powder, 0.5-1.5 units/mg protein (using chondroitin sulfate A as substrate)
foreign activity
Glycosaminoglycan (GAG) degradation enzymes may contain trace amount
form
lyophilized powder
MDM Code
C2780-5UN
Material Number
C2780
Business Unit
760
Shipping Mode
AIR
MRP Type
PD
Local HS Code
35079000
Legacy
SIGMA
Plant
6311
Sales Org
6304
CAS Number
9047-57-8
Description
Application
Chondroitinase AC from Sigma has been used for the large scale preparation of glycosaminoglycan (GAG) fractions during the study of structural and sequence motifs in dermatan sulfate.
Biochem/physiol Actions
Chondroitinase AC from Flavobacterium heparinum is an enzyme that cleaves sulfated and non-sulfated polysaccharide chains with (1-4) linkages between hexosamines and glucuronic acid residues, by an elimination mechanism. The resulting oligosaccharide products are mainly disaccharides with unsaturated uronic acids. Chondroitinase AC specifically degrades chondroitin sulfates A and C, but not chondroitin sulfate B (dermatan sulfate).
Other Notes
Contains potassium phosphate buffer salts and BSA as stabilizer.
Preparation Note
Reconstitute the product in 20 mM phosphate buffer, pH 7.0. Subsequent dilutions can be made with a 0.01% aqueous bovine serum albumin solution.
Suitability
Depending on reaction conditions, digestion of chondroitin sulfate may result in hydrolysis of disaccharides
Unit Definition
One unit will cause a Δ
Warning
A232 of 1.0 per minute due to the release of unsaturated disaccharide from chondroitin sulfate A at pH 7.3 at 37 °C. Reaction volume: 3.1 ml (light path 1 cm).
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