C2780
Sigma-Aldrich

Chondroitinase AC from Flavobacterium heparinum

Nacres: AC from Flavobacterium heparinum
CAS Number: 9047-57-8
Grouped product items
SKU Pack Size Availability Price Quantity
C2780-5UN 5UN Available to ship on February 22, 2024
IDR5,771,000.00
Request a Bulk Order Check Availability

Properties

Properties
composition Protein ~15% Lowry
Description Chondroitinase AC from Flavobacterium heparinum lyophilized powder, 0.5-1.5 units/mg protein (using chondroitin sulfate A as substrate)
foreign activity Glycosaminoglycan (GAG) degradation enzymes may contain trace amount
form lyophilized powder
MDM Code C2780-5UN
Material Number C2780
Business Unit 760
Shipping Mode AIR
MRP Type PD
Local HS Code 35079000
Legacy SIGMA
Plant 6311
Sales Org 6304
CAS Number 9047-57-8

Description

Application

Chondroitinase AC from Sigma has been used for the large scale preparation of glycosaminoglycan (GAG) fractions during the study of structural and sequence motifs in dermatan sulfate.

Biochem/physiol Actions

Chondroitinase AC from Flavobacterium heparinum is an enzyme that cleaves sulfated and non-sulfated polysaccharide chains with (1-4) linkages between hexosamines and glucuronic acid residues, by an elimination mechanism. The resulting oligosaccharide products are mainly disaccharides with unsaturated uronic acids. Chondroitinase AC specifically degrades chondroitin sulfates A and C, but not chondroitin sulfate B (dermatan sulfate).

Other Notes

Contains potassium phosphate buffer salts and BSA as stabilizer.

Preparation Note

Reconstitute the product in 20 mM phosphate buffer, pH 7.0. Subsequent dilutions can be made with a 0.01% aqueous bovine serum albumin solution.

Suitability

Depending on reaction conditions, digestion of chondroitin sulfate may result in hydrolysis of disaccharides

Unit Definition

One unit will cause a Δ

Warning

A232 of 1.0 per minute due to the release of unsaturated disaccharide from chondroitin sulfate A at pH 7.3 at 37 °C. Reaction volume: 3.1 ml (light path 1 cm).

SAFETY INFORMATION

Signal Word

RIDADRRIDADR

WGK Germany

WGK 3

Flash Point (F)

Not applicable

Flash Point (C)

Not applicable

Target Organs

Risk Statement

Supplemental Hazard Statements

DOCUMENTATION

Certificate of Analysis View Sample COA
Certificate of Origin

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