[3:39 PM] Rifki Akbar
C2905
Sigma-Aldrich

CHONDROITINASE ABC FROM PROTEUS VULGARIS

Nacres: ABC from Proteus vulgaris
Pubchem Substance Id: 232-777-9
CAS Number: 9024-13-9
Grouped product items
SKU Pack Size Availability Price Quantity
C2905-5UN - Available to ship on May 02, 2024
IDR 4,553,000.00
C2905-10UN - Available to ship on May 02, 2024
IDR 7,858,000.00
C2905-2UN - Available to ship on May 02, 2024
IDR 2,286,000.00
Request a Bulk Order Check Availability

Properties

Properties
applications diagnostic assay manufacturing
biological source bacterial (Proteus vulgaris)
composition Protein ~35% biuret
Description CHONDROITINASE ABC FROM PROTEUS VULGARIS
form lyophilized powder
MDM Code C2905-5UN
Material Number C2905
Business Unit 760
Shipping Mode AIR
MRP Type PD
Local HS Code 35079000
Legacy SIGMA
Plant 6311
Sales Org 6304
CAS Number 9024-13-9

Description

Application

Chondroitinase ABC from Sigma has been used to digest O-linked chondroitin sulfate-like glycosaminoglycan (CSGAG). The enzyme has also been used to digest glycosaminoglycan chains in proteoglycans during the construction and expression of a recombinant versican gene in mammalian systems. Furthermore, it has been used to digest chondroitin sulfate in the proteoglycan complexes. The enzyme has also been used in the removal of glycan chains from aggrecan, which eliminates the axon growth-inhibitory effect of aggrecan.

Biochem/physiol Actions

Chondroitinase ABC catalyzes the eliminative degradation of polysaccharides containing (1-4)-β-D-hexosaminyl and (1-3)-β-D-glucuronosyl (or (1-3)-α-L-iduronosyl) linkages to disaccharides containing 4-deoxy-β-D-gluc-4-enuronosyl groups. It acts on chondroitin 4-sulfate, chondroitin 6-sulfate, and dermatan sulfate. However, it acts slowly on hyaluronate. The molecular weight is approximately 120 kDa with 2 non-identical subunits of molecular masses 86 kDa and 32 kDa. The pH optimum is 8.0 with chondroitin sulfate and 6.8 with hyaluronic acid. The optimal temperature is 37 °C. 1 mM Zn2+ acts as an inhibitor, while 0.05 M acetate is an activator of the enzyme.

Packaging

Packages based on chondroitinase C activity.

Physical form

Lyophilized powder containing Tris buffer salts

Preparation Note

Reconstitute in a 0.01% bovine serum albumin aqueous (BSA) solution. Subsequent dilutions can be made into a buffer containing 50 mM Tris, pH 8.0, with 60 mM sodium acetate and 0.02% BSA. Solutions should be freshly prepared.

Unit Definition

One unit will liberate 1.0 μmole of a mixture of 2-acetamido-2-deoxy-3-O-(β-D-gluc-4-ene-pyranosyluronic acid)-4-O-sulfo-D-galactose and 1.0 μmole of 2-acetamido-2-deoxy-3-O-(β-D-gluc-4-ene-pyranosyluronic acid)-6-O-sulfo-D-galactose from chondroitin sulfate from shark cartilage, per min at pH 8.0 at 37 °C.

SAFETY INFORMATION

Signal Word

RIDADRRIDADR

WGK Germany

WGK 3

Flash Point (F)

Not applicable

Flash Point (C)

Not applicable

Target Organs

Risk Statement

Supplemental Hazard Statements

DOCUMENTATION

Certificate of Analysis View Sample COA
Certificate of Origin

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