C8487
Sigma-Aldrich

ANTI-CASPASE 3, ACTIVE, DEVELOPED IN RAB

Synonyms English:

Activated Caspase 3 Antibody; Activated Caspase 3 Antibody - Anti-Caspase 3, Active antibody produced in rabbit; Anti-Apopain; Anti-CPP32; Anti-Yama

Nacres: NA.41
Mdl No: MFCD01321906
Grouped product items
SKU Pack Size Availability Price Quantity
C8487-100UL - Available to ship on April 04, 2024
IDR 7,696,000.00
C8487-200UL - Available to ship on April 04, 2024
IDR 9,952,000.00
C8487-25UL - Available to ship on April 04, 2024
IDR 3,227,000.00
Request a Bulk Order Check Availability

Properties

Properties
antibody form IgG fraction of antiserum
antibody product type primary antibodies
biological source rabbit
clone polyclonal
conjugate unconjugated
Description ANTI-CASPASE 3, ACTIVE, DEVELOPED IN RAB
form buffered aqueous solution
gene information human ... CASP3(836)
mouse ... Casp3(12367)
rat ... Casp3(25402)
MDM Code C8487-100UL
Material Number C8487
Business Unit 696
Shipping Mode AIR
MRP Type PD
Local HS Code 30021500
Legacy SIGMA
Plant 6311
Sales Org 6304

Description

Application

Anti-Caspase 3, Active antibody has been used:
  • for western blotting of cytochrome c for caspase activation
  • as primary antibody in immunofluorescence staining of embryos and postnatal mice cryosections
  • in Western blot analysis of activated caspase 3
  • as a primary antibody in immunodetection of rat brain sections

Biochem/physiol Actions

Caspase 3 is one of the key effectors of apoptosis. It acts downstream of caspase 9 in the apoptotic pathway. During apoptosis, it gets activated by proteolytic cleavage into the 17-19 kDa (p17, p18) and 12 kDa (p12) active subunits. Any alteraion in CASP-3 in mice, causes premature lethality. It has also shown that the deletion of gene affects brain development resulting of hyperplasias and disorganized cell deployement. Clinically it is related to Huntington disease (HD). Caspase 3 brings about cell death by activating CAD (caspase activated DNAse I), an endonuclease that causes degradation of chromosomal DNA.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

Caspase-3 (CASP3) belongs to the class of cysteine proteases. CASP3 is an executioner caspase, which is cleaved and activated with the help of caspase-8 and caspase-9 initiator caspases. CASP3 is mapped to human chromosome 4q35.1.

Immunogen

Synthetic peptide corresponding to the cleavage site of human caspase 3 conjugated to KLH.

Physical form

Supplied in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Storage and Stability

Store at –20 °C. For continuous use, the product may be storage, the product may be stored at 2-8 °C for up to one month. For prolonged storage, freeze in working aliquots at –20 °C. Repeated freezing and thawing, or storage in frost-free freezers, is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilutions should be discarded if not used within 12 hours.

SAFETY INFORMATION

Signal Word

Warning

P273 - P280 - P302 + P352 + P312 - P362 + P364 - P501

RIDADRRIDADR

WGK Germany

WGK 1

Flash Point (F)

Not applicable

Flash Point (C)

Not applicable

Target Organs

Risk Statement

Supplemental Hazard Statements

DOCUMENTATION

Certificate of Analysis View Sample COA
Certificate of Origin

TECHNICAL SERVICE

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.