Dispase(R) II

Nacres: II
Pubchem Substance Id: 255-914-4
Grouped product items
SKU Pack Size Availability Price Quantity
D4693-1G 1G Available to ship on February 22, 2024
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Description Dispase(R) II protease
form lyophilized solid
MDM Code D4693-1G
Material Number D4693
Business Unit 760
Shipping Mode AIR
Local HS Code 35079000
Legacy SIGMA
Plant 6311
Sales Org 6304


Analysis Note

The enzyme can be initially dissolved in 50 mM Hepes/KOH pH 7.4, 150 mM NaCl at 10 mg/ml. Further dilutions should be made in the cell culture media most appropriate for the cells being dissociated. Dispase is a metalloproteinase and is activated by divalent cations including Ca2+ , Mg2+, Mn2+ and Fe2+.


The enzyme from Sigma has been used in developing a protocol for ex vivo culture of mouse embryonic mammary buds. It has been used in the treatment of rat heart pieces during the isolation of mitochondria from rat heart. It has also been used for the isolation of dental pulp stem cells (DPSCs) by enzymatic hydrolysis. These cells have been compared with DPSCs isolated by explant method to analyse their stem cell and differentiation properties.

Biochem/physiol Actions

II has been used for Fluorescence-Activated Cell Sorting (FACS). It has also been used for separating visceral yolk sac layers.


Dispase® II is a neutral protease that hydrolyzes the N-terminal peptide bonds of non-polar amino acid residues. It may be used for separating many tissues and cells grown in vitro. The enzyme is very gentle and does not damage cell membranes. It can also be used to prevent clumping in suspension cultures. This protease cleaves fibronectin and type IV collagen, but not laminin, type V collagen, serum albumin, or transferrin. Dispase® II is specific for the cleavage of Leucine-Phenylalanine bonds. Ca2+, Mg2+, Mn2+, Fe2+, Fe3+ and Al3+ activate the enzyme. EDTA, EGTA, Hg2+ and other heavy metals inhibit the enzyme activity. The enzyme contains 1g-atom of zinc per g-mol of purified enzyme. If this zinc component is removed by chelating agents such as EDTA or EGTA, an inactive apoenzyme is obtained. The enzyme is not inhibited by serum.

Physical properties

lyophilized powder containing calcium acetate and milk sugar


A 50 U/mL stock solution may be prepared by dissolving the powder in a buffer containing 10 mM NaAc (pH 7.5) and 5 mM CaAc. It should be stored at 4 °C.


One unit will hydrolyze casein to produce color equivalent to 1.0 μmole (181 μg) of tyrosine per min at pH 7.5 at 37 °C (color by Folin-Ciocalteu reagent), unless otherwise indicated.



Signal Word


H315 - H319 - H334 - H335

P261 - P264 - P271 - P280 - P302 + P352 - P305 + P351 + P338


WGK Germany


Flash Point (F)

Not applicable

Flash Point (C)

Not applicable

Target Organs

Respiratory system

Risk Statement

Supplemental Hazard Statements


Certificate of Analysis View Sample COA
Certificate of Origin


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