D8418
Sigma-Aldrich

Dimethyl sulfoxide

Linear Formula:

(CH3)2SO

Empform:

C2H6OS

Synonyms English:

Methyl Sulfoxide

Nacres: NA.52
Mdl No: MFCD00002089
Fw: 78.13
Pubchem Substance Id: 24894214
CAS Number: 67-68-5
Grouped product items
SKU Pack Size Availability Price Quantity
D8418-100ML 1X100mL Available to ship on March 28, 2024
IDR 1,385,000.00
D8418-500ML 500ML Available to ship on March 28, 2024
IDR 4,055,000.00
D8418-50ML 50ML Available to ship on March 28, 2024
IDR 812,000.00
D8418-250ML 250ML Available to ship on March 28, 2024
IDR 2,740,000.00
D8418-1L - Available to ship on March 28, 2024
IDR 5,882,000.00
Request a Bulk Order Check Availability

Properties

Properties
Description Dimethyl sulfoxide for molecular biology
MDM Code D8418-500ML
Material Number D8418
Business Unit 042
Shipping Mode AIR
MRP Type PD
Local HS Code 29309090
Legacy SIGMA
Plant 6311
Sales Org 6304
CAS Number 67-68-5

Description

Application

DMSO (dimethyl sulfoxide) is a highly polar, aprotic organic solvent with many applications in organic chemistry and molecular biology. DMSO is routinely used in

  • polymerase chain reaction (PCR)
  • amplification of cDNA libraries
  • DNA sequencing
  • column-loading buffers for poly(A)+ RNA selection
  • buffers for the transformation of competent E. coli
  • transfection protocols.

Dimethyl sulfoxide has been used-

  • as an oligonucleotide solvent in array spotting[1]
  • as a solvent during miRNA microarray analysis and quantitative real-time PCR (qRT-PCR)
  • for cell lysis during MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) assay
  • for the preparation of the stock solution during MMP (mitochondrial membrane potential)cytofluorimetric assessment
  • in the storage of human and animal cell lines and bacteriophage λ as a cryoprotective agent.
To prepare a sterile filtered DMSO solution, it is recommended to use a PTFE or nylon membrane. Cellulose acetate membranes are not recommended.

Caution

Supercools easily and remelts slowly at room temperature. Solidified product can be re-liquified by warming to room temperature without detriment to the product.

Features and Benefits

This product is free from DNases, RNases, phosphatases and proteases.

General description

Features and Benefits:

  • Suitable for Molecular Applications
  • Nuclease-Free DMSO
  • DNase-Free, RNase-Free, Protease-Free, Phosphatase-Free
  • Molecular Biology Grade DMSO

Dimethyl sulfoxide (DMSO) is a highly polar organic reagent that has exceptional solvent properties for organic and inorganic chemicals.
This product is designated as Molecular Biology grade and is suitable for molecular biology applications. It has been analyzed for the presence of nucleases and proteases.

SAFETY INFORMATION

Signal Word

RIDADRRIDADR

WGK Germany

WGK 1

Flash Point (F)

188.6 °F - closed cup

Flash Point (C)

87 °C - closed cup

Target Organs

Risk Statement

Supplemental Hazard Statements

DOCUMENTATION

Certificate of Analysis View Sample COA
Certificate of Origin

TECHNICAL SERVICE

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