[3:39 PM] Rifki Akbar
D9542
Sigma-Aldrich

DAPI

Empform:

C16H15N5 · 2HCl

Synonyms English:

4′,6-Diamidino-2-phenylindole dihydrochloride; 2-(4-Amidinophenyl)-6-indolecarbamidine dihydrochloride; DAPI dihydrochloride

Nacres: NA.52
Mdl No: MFCD00012681
Fw: 350.25
Pubchem Substance Id: 24894305
Grouped product items
SKU Pack Size Availability Price Quantity
D9542-5MG - Available to ship on April 26, 2024
IDR 1,019,000.00
D9542-10MG - Available to ship on April 26, 2024
IDR 1,580,000.00
D9542-1MG - Available to ship on April 26, 2024
IDR 205,000.00
D9542-50MG - Available to ship on April 26, 2024
IDR 6,849,000.00
Request a Bulk Order Check Availability

Properties

Properties
Epsilon extinction coefficient 30 at 263 
Lambda max nm in H2O at 1 
Lambda min mM
astm d 2162
astm d 301-56 98% (HPLC and TLC)
Description DAPI
free fatty acid content2 nm (nur DAPI)
foreign activity λ
form ex 
format 340 
fragrance allergen nm
frame l × w λ
frame size em 
frame w × l 488 
frit diam. × h powder
growth mode for molecular biology
inlet diam. 1S/C16H15N5.2ClH/c17-15(18)10-3-1-9(2-4-10)13-7-11-5-6-12(16(19)20)8-14(11)21-13
inlet nptf /h1-821H(H31718)(H31920)
inlet o.d. 2*1H
inlet pressure measuring range FPNZBYLXNYPRLR-UHFFFAOYSA-N
MDM Code D9542-10MG
Material Number D9542
Business Unit 710
Shipping Mode AIR
MRP Type YP
Local HS Code 29339990
Legacy SIGMA
Plant 6311
Sales Org 6304

Description

Application

DAPI is several times more sensitive than ethidium bromide for staining DNA in agarose gels. It may be used for photofootprinting of DNA, to detect annealed probes in blotting applications by specifically visualizing the double-stranded complex, and to study the changes in DNA and analyze DNA content during apoptosis using flow cytometry. DAPI staining has also been shown to be a sensitive and specific detection method for mycoplasma.

Biochem/physiol Actions

Cell permeable fluorescent minor groove-binding probe for DNA. Binds to the minor groove of double-stranded DNA (preferentially to AT rich DNA), forming a stable complex which fluoresces approximately 20 times greater than DAPI alone.

Caution

Protect from light.

General description

DAPI (4′,6-Diamidino-2-phenylindole dihydrochloride) is a cell permeable, fluorescent dye that binds to DNA.

SAFETY INFORMATION

Signal Word

Warning

H315 - H317 - H335

P261 - P264 - P271 - P272 - P280 - P302 + P352

RIDADRRIDADR

WGK Germany

WGK 3

Flash Point (F)

Not applicable

Flash Point (C)

Not applicable

Target Organs

Respiratory system

Risk Statement

Supplemental Hazard Statements

DOCUMENTATION

Certificate of Analysis View Sample COA
Certificate of Origin

TECHNICAL SERVICE

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