[3:39 PM] Rifki Akbar
E6909
Sigma-Aldrich

ECM GEL, GROWTH FACTOR REDUCED FROM ENG&

Nacres: NA.75
Mdl No: MFCD00283131
Grouped product items
SKU Pack Size Availability Price Quantity
E6909-10ML - Please Contact our customer service for price and availability
IDR 13,003,000.00
E6909-5ML - Please Contact our customer service for price and availability
IDR 7,423,000.00
Request a Bulk Order Check Availability

Properties

Properties
components ECM was prepared to a protein concentration of 8-12 mg/mL, containing laminin as a major component, collagen type IV, heparin sulfate proteoglycan, entactin, and other minor components. Addition of collagen type IV to the ECM gel increases polymerization.
The growth factor reduction modification allows for more control of medium, better interpretation of results and increased polymerization. The medium also lacks phenol red, which can interfere with some analyses.
concentration 7-9 
concentricity mg/mL
Description ECM GEL, GROWTH FACTOR REDUCED FROM ENG&
format liquid
glass tube l × o.d. × i.d. mouse ... Hspg2(15530) Lama1(16772) Lama3(16774) Lama5(16776) Lamb1-1(16777) Lamc1(226519) Lamc2(16782) Nid1(18073) Sdc2(15529)
MDM Code E6909-10ML
Material Number E6909
Business Unit 768
Shipping Mode COU
MRP Type PD
Local HS Code 38210010
Legacy SIGMA
Plant 6311
Sales Org 6304

Description

Application

ECM Gel from Engelbreth-Holm-Swarm murine sarcoma has been used to coat tissue culture plates for different glioma subtypes and C2C12 muscle cells. It has also been used as a scaffold to increase myotube attachment, maturation and survival.

Biochem/physiol Actions

The ECM gel will undergo thermally activated polymerization when brought to 20-40°C, forming a reconstituted basement membrane. PC12 cells will show neurite formation within 2 days when they are grown on a thin layer of this ECM gel.

Caution

For long term storage, keep product at -20°C. The ECM gel may be stored at 2-8°C for up to 72 hours.

Preparation Note

This product is prepared from Engelbreth-Holm-Swarm sarcoma in mice; dialyzed against chloroform. Gel should be thawed overnight at 2-8°C before use and dispensed to a multiwall plate using a plate and pipettes that are pre-cooled to 2-8°C. The gel may contain precipitates which do not influence its activity. The gel may also be diluted up to twofold with 2-8°C Dulbecco′s Modified Eagle′s Medium, and should be done before gel is added to the plate. The product will gel within 5 minutes at 20°C. For prolonged manipulations, work should be conducted below 10°C. Cells can be plated on top of a thin gel layer of 0.5 mm or cultured inside a 1 mm layer. If you culture cells inside, add the cells to the gel prior to plating at a recommended density of 3-4 x 104 cells per mL. To dissociate cells from this gel, use protease in PBS without calcium, magnesium, or EDTA, at a concentration of 0.6-2.4 units per mL.

SAFETY INFORMATION

Signal Word

RIDADRRIDADR

WGK Germany

WGK 3

Flash Point (F)

Not applicable

Flash Point (C)

Not applicable

Target Organs

Risk Statement

Supplemental Hazard Statements

DOCUMENTATION

Certificate of Analysis View Sample COA
Certificate of Origin

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