G3272
Sigma-Aldrich

GUANIDINE HYDROCHLORIDE

Linear Formula:

NH2C(=NH)NH2 · HCl

Empform:

CH5N3 · HCl

Synonyms English:

Aminoformamidine hydrochloride

Nacres: NA.31
Mdl No: MFCD00013026
Fw: 95.53
Pubchem Substance Id: 24895119
Grouped product items
SKU Pack Size Availability Price Quantity
G3272-100G 100g Available to ship on October 21, 2023
IDR1,959,000.00
G3272-500G 500G Available to ship on October 21, 2023
IDR6,279,000.00
G3272-2KG 2KG Available to ship on October 21, 2023
IDR19,903,000.00
G3272-1KG 1KG Available to ship on October 21, 2023
IDR11,004,000.00
G3272-25G - Available to ship on October 21, 2023
IDR1,075,000.00
Request a Bulk Order Check Availability

Properties

Properties
assay
assay range 99%
blade diam. synthetic (organic)
cavity d Pb: ≤
cavity diam.
cavity diam. × d ppm
column d × l white
depth × h × w 1.3 
depth × overall h g/cm3 (lit.)
frame l × w DNase RNase none detected
frame size crystalline powder
gloss human ... KCNA1(3736) KCNA10(3744) KCNA2(3737) KCNA3(3738) KCNA4(3739) KCNA5(3741) KCNA6(3742) KCNA7(3743) KCNB1(3745) KCNB2(9312) KCNC1(3746) KCNC2(3747) KCNC3(3748) KCNC4(3749) KCND1(3750) KCND2(3751) KCND3(3752) KCNF1(3754) KCNG1(3755) KCNG2(26251) KCNG3(170850) KCNG4(93107) KCNH1(3756) KCNH2(3757) KCNH3(23416) KCNH4(23415) KCNH5(27133) KCNH6(81033) KCNH7(90134) KCNH8(131096) KCNQ1(3784) KCNQ2(3785) KCNQ3(3786) KCNQ4(9132) KCNQ5(56479) KCNS1(3787) KCNS2(3788) KCNS3(3790) KCNV1(27012) KCNV2(169522)
granulation for molecular biology
inherent viscosity
inlet and outlet diam. 0.3% water (Karl Fischer)
inlet and outlet nptm × nptm 1S/CH5N3.ClH/c2-1(3)4
inlet and outlet size /h(H5234)
inlet cga 1H
inlet connection diam. PJJJBBJSCAKJQF-UHFFFAOYSA-N
MDM Code G3272-100G
Material Number G3272
Business Unit 499
Shipping Mode AIR
MRP Type PD
Local HS Code 29252900
Legacy SIGMA
Plant 6311
Sales Org 6304

Description

Application

Strong chaotropic agent useful for the denaturation and subsequent refolding of proteins. This strong denaturant can solubilize insoluble or denatured proteins such as inclusion bodies. This can be used as the first step in refolding proteins or enzymes into their active form. Urea and dithiothreitol (DTT) may also be necessary.

Biochem/physiol Actions

Guanidine hydrochloride (GuHCl) is a small hydroscopic molecule. It plays a role in inhibiting heat shock protein 104 (Hsp104) adenosine triphosphatase (ATPase) activity in vivo. It is a potent denaturant and inactivator of several enzymes and proteins. GuHCl can inactivate aminoacylase and papain. It is involved in unfolding and inactivation of alkaline phosphatase in Haliotis diversicolor. At higher concentrations, GuHCl is involved in virus inactivation such as Herpes simplex virus 1 (HSV-1).

Caution

May agglomerate upon storage. The quality of the product does not appear to be affected and solutions prepared from the free-flowing and lumpy guanidine hydrochloride appear identical.

Preparation Note

In order to make an 8M solution in water, one must heat the solution to 35 °C for approximately 30 minutes.
The maximum solubility of guanidine hydrochloride in water at room temperature is approximately 6M.

SAFETY INFORMATION

Signal Word

Warning

H302 + H332 - H315 - H319

P261 - P264 - P301 + P312 - P302 + P352 - P304 + P340 + P312 - P305 + P351 + P338

RIDADRRIDADR

WGK Germany

WGK 1

Flash Point (F)

Not applicable

Flash Point (C)

Not applicable

Target Organs

Risk Statement

Supplemental Hazard Statements

DOCUMENTATION

Certificate of Analysis View Sample COA
Certificate of Origin

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