Tumor Necrosis Factor-alpha human TNF-alpha, recombinant, expressed in HEK 293 cells, suitable for cell culture
format
lyophilized powder
glass tube l × o.d. × i.d.
human ... TNF(7124)
inchi
≤
inchi key
1
indiff. to chromic acid
EU/μ
inherent viscosity
g
MDM Code
H8916-10UG
Material Number
H8916
Business Unit
694
Shipping Mode
COU
MRP Type
PD
Local HS Code
30021500
Legacy
SIGMA
Plant
6311
Sales Org
6304
CAS Number
94948-59-1
Description
Analysis Note
The specific activity was determined by the dose-dependent cytotoxity of the TNF alpha sensitive cell line L-929 in the presence of actinomycin D.
Application
Tumor Necrosis Factor-α
Biochem/physiol Actions
(TNF-α
Caution
) human has been used
In induction of netting neutrophils by anti-neutrophil cytoplasmic antibody and to study its effect on platelet activation and formation of monomeric C-reactive protein.
To study the effect of TNF-α
Cell Line Description
on miR-221 and fractalkine expression.
To induce inflammatory cell responses.
In NF-κ
Cell Line Origin
B luciferase reporter assay.
Chem/Phys Resistance
Tumor Necrosis Factor-α (TNF-α) is a potent pro-inflammatory cytokine that plays a role in the rheumatoid arthritis pathology, psoriatic arthritis (PsA) and psoriasis. It stimulates interleukins IL-1 and IL-6. Polymorphism in the TNF-α gene is associated with destructive arthropathy in PsA. The post-translational modifications in TNF-a is crucial for its functionality.
Other Notes
The TNFα
Packaging
(tumor necrosis factor α
Physical form
) gene is mapped to human chromosome 6p21.33. TNFα
Physical properties
is a member of TNF superfamily. TNF-α
Preparation Note
has a palmitoyl group in the cysteine residue and is phosphorylated in the transmembrane region serine residue.
Serological Profile
Tumor Necrosis Factor-α (TNF-α) is expressed in human HEK 293 cells and has been shown to be predominantly a glycosylated, non-covalently linked homotrimer with a molecular mass of 51 kDa (gel filtration). Production in human 293 cells offers authentic glycosylation. Glycosylation contributes to stability in cell growth media and other applications.
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