[3:39 PM] Rifki Akbar
KCQS07
Sigma-Aldrich

KICQSTART 1-STEP PROBE RT-QPCR MIX

Nacres: One-Step Probe RT-qPCR ReadyMix
Grouped product items
SKU Pack Size Availability Price Quantity
KCQS07-500RXN - Available to ship on May 18, 2024
IDR 11,690,000.00
Request a Bulk Order Check Availability

Properties

Properties
compatibility Bio-Rad CFX384
Description KICQSTART 1-STEP PROBE RT-QPCR MIX
detection method probe-based
feature dNTPs included
form liquid
input purified RNA
MDM Code KCQS07-500RXN
Material Number KCQS07
Shipping Mode AIR
MRP Type PD
Local HS Code 38229090
Legacy SIGMA
Plant 6311
Sales Org 6304

Description

Application

KiCqStart®

Biochem/physiol Actions

One-Step Probe RT-qPCR ReadyMix

Caution

has been used:
  • in quantitative real-time polymerase chain reaction (qRT-PCR) for gene expression studies
  • in cell type-specific transcript analysis for determining the cycle threshold (Ct) value
  • for severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) RNA detection from nasopharyngeal and oropharyngeal swabs
  • for amplification of reverse-transcribed cDNA

DNA Profile

2X reaction buffer containing dATP, dCTP, dGTP, dTTP, magnesium chloride, reverse transcriptase, RNase inhibitor protein, hot-start DNA polymerase, inert blue qPCR dye, and stabilizers

packaging:
500 reactions* = 5 X 1 mL tubes
*number of reactions based on a 20uL volume

Formula variant

  • Assay results in as little as 33 minutes
  • Highly efficient and sensitive real-time PCR results
  • Little/no optimization required

Physical form

Different real-time PCR systems employ different strategies for normalization of fluorescent signals and correction of well-to-well optical variations. It is critical to match the appropriate qPCR reagent to your specific instrument.KiCqStart®

Physical properties

One-Step Probe RT-qPCR ReadyMix

Preparation Note

does not contain an internal reference dye. Compatible instruments include:
  • Bio-Rad®

Principle

CFX384

Publishing

  • Bio-Rad CFX96
  • Quality

  • Bio-Rad MiniOpticon
  • Quantity

  • Bio-Rad/MJ Chromo4
  • Reconstitution

  • Cepheid SmartCycler®
  • Sequence

  • Eppendorf Mastercycler®
  • Serological Profile

    ep realplex
  • Eppendorf Mastercycler ep realplex2 s
  • Illumina Eco qPCR
  • Qiagen/Corbett Rotor-Gene®
  • Specifications

    3000
  • Qiagen/Corbett Rotor-Gene 6000• Qiagen/Corbett Rotor-Gene Q
  • Roche LightCycler®
  • Specificity

    480

    SAFETY INFORMATION

    Signal Word

    RIDADRRIDADR

    WGK Germany

    WGK 3

    Flash Point (F)

    Not applicable

    Flash Point (C)

    Not applicable

    Target Organs

    Risk Statement

    Supplemental Hazard Statements

    DOCUMENTATION

    Certificate of Analysis View Sample COA
    Certificate of Origin

    TECHNICAL SERVICE

    Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.