ATP Assay Kit Sufficient for 100 Colorimetric or Fluorometric tests
MDM Code
MAK190-1KT
Material Number
MAK190
Business Unit
777
Shipping Mode
AIR
MRP Type
PD
Local HS Code
38229090
Legacy
SIGMA
Plant
6311
Sales Org
6304
Description
Application
ATP Assay Kit has been used to quantify the ATP concentration by fluorometric detection of ATP in samples.
General description
Adenosine triphosphate (ATP) is a nucleoside triphosphate formed exclusively in the mitochondria. It is a high-energy molecule described as the energy currency in all living systems. The chemical energy contained in the phosphate bond of ATP drives most cellular processes. Genetic disorders such as Leber hereditary optic neuropathy, Leigh syndrome, neuropathy, ataxia, and retinitis pigmentosa affect the generation of ATP in the mitochondria.
Other Notes
The fluorometric assay is 10-100 times more sensitive than the colorimetric assay.
Principle
The ATP Colorimetric/Fluorometric Assay Kit is a sensitive assay for determining ATP in various samples (ranging from 2–10 nmole/well for the colorimetric assay and from 20–1,000 pmole/well for the fluorometric assay). ATP concentration is determined by phosphorylating glycerol, resulting in a colorimetric (570 nm) or fluorometric (lex = 535/lem = 587 nm) product proportional to the amount of ATP present.
Suitability
Suitable for the determination of ATP in samples such as animal tissue and cell culture samples.
Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.
