[3:39 PM] Rifki Akbar
MBD0020
Sigma-Aldrich

MBD0020-10ML-PH

Empform:

C16H15N5 · 2HCl

Synonyms English:

4′,6-Diamidino-2-phenylindole dihydrochloride; DAPI Dye; DAPI Stain; DNA Content Counterstain; 2-(4-Amidinophenyl)-6-indolecarbamidine dihydrochloride; DAPI dihydrochloride

Mdl No: MFCD00012681
Fw: 350.25
Grouped product items
SKU Pack Size Availability Price Quantity
MBD0020-10ML MBD0020-10ML Available to ship on May 11, 2024
IDR 2,322,000.00
Request a Bulk Order Check Availability

Properties

Properties
fluorescence λ
foil thickness ex 
food allergen 340 
foreign activity nm
form λ
format em 
fragrance allergen 488 
frame l × w nm (nur DAPI)
free fatty acid content content liquid
inlet cga 1S/C16H15N5.2ClH/c17-15(18)10-3-1-9(2-4-10)13-7-11-5-6-12(16(19)20)8-14(11)21-13
inlet connection size /h1-821H(H31718)(H31920)
inlet diam. 2*1H
inlet i.d. FPNZBYLXNYPRLR-UHFFFAOYSA-N
MDM Code MBD0020-10ML
Material Number MBD0020
Business Unit 042
Shipping Mode AIR
MRP Type PD
Local HS Code 38249999
Legacy SIGMA
Plant 6311
Sales Org 6304

Description

Application

DAPI Ready Made Solution with Antifade is a glycerol based ready to use solution provided at 1μ

Biochem/physiol Actions

g/mL concentration in a dropper vial that can be applied directly to cells or tissue samples on microscope slides or coverslips. It is recommended to warm up at room temperature and mix by swirling the dropper vial before use. Store DAPI Ready Made Solution with antifade at -20 C protected from light.

Caution

Cell permeable fluorescent minor groove-binding probe for DNA. Binds to the minor groove of double-stranded DNA (preferentially to AT rich DNA), forming a stable complex which fluoresces approximately 20 times greater than DAPI alone.

Footnote

  • Protects dyes from fading during imaging.
  • Ready-to-use liquid.
  • Easy to use Dropper vial application.
  • Stained sample are stable for longer time period.
  • Maintains fluorescence signal-little to no quenching.

Immunogen

DAPI, 4′,6-diamidino-2-phenylindole, is a blue fluorescent nucleic acid stain that fluoresces brightly upon selectively binding to the minor groove of double stranded DNA. Its selectivity for DNA and high cell permeability allows efficient staining of nuclei with little background from the cytoplasm. The excitation and emission wavelengths of DAPI-DNA complex are 360 nm and 460 nm, respectively. Most of the fluorescent dyes fade (photobleach) when exposed to excitation light. The amount of photobleaching depends on the composition of the molecule and the time of light exposure. DAPI Ready Made Solution with Antifade is a formulation that suppress photobleaching and prolong fluorescent signals.

Physical properties

Liquid - DAPI readymade with antifade solution is provided at 1μ

Preparation Note

g/mL concentration in glycerol-PBS based solution.

SAFETY INFORMATION

Signal Word

RIDADRRIDADR

WGK Germany

WGK 2

Flash Point (F)

Not applicable

Flash Point (C)

Not applicable

Target Organs

Risk Statement

Supplemental Hazard Statements

DOCUMENTATION

Certificate of Analysis View Sample COA
Certificate of Origin

TECHNICAL SERVICE

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