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    • MBD0020-10ML-PH

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    Documents SDS COO/COA Specification Sheet
    MBD0020
    Sigma-Aldrich

    MBD0020-10ML-PH

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    Empform:

    C16H15N5 · 2HCl

    Synonyms English:

    4′,6-Diamidino-2-phenylindole dihydrochloride; DAPI Dye; DAPI Stain; DNA Content Counterstain; 2-(4-Amidinophenyl)-6-indolecarbamidine dihydrochloride; DAPI dihydrochloride

    Mdl No: MFCD00012681
    Fw: 350.25
    Grouped product items
    SKU Pack Size Availability Price Quantity
    MBD0020-10ML MBD0020-10ML Available to ship on June 25, 2023
    IDR2,322,000.00
    Request a Bulk Order Check Availability
    Documents SDS COO/COA Specification Sheet

    Properties

    Properties
    fluorescence λ
    foil thickness ex 
    food allergen 340 
    foreign activity nm
    form λ
    format em 
    fragrance allergen 488 
    frame l × w nm (nur DAPI)
    free fatty acid content content liquid
    inlet cga 1S/C16H15N5.2ClH/c17-15(18)10-3-1-9(2-4-10)13-7-11-5-6-12(16(19)20)8-14(11)21-13
    inlet connection size /h1-821H(H31718)(H31920)
    inlet diam. 2*1H
    inlet i.d. FPNZBYLXNYPRLR-UHFFFAOYSA-N
    MDM Code MBD0020-10ML
    Material Number MBD0020
    Business Unit 042
    Shipping Mode AIR
    MRP Type PD
    Local HS Code 38249999
    Legacy SIGMA
    Plant 6311
    Sales Org 6304

    Description

    Application

    DAPI Ready Made Solution with Antifade is a glycerol based ready to use solution provided at 1μ

    Biochem/physiol Actions

    g/mL concentration in a dropper vial that can be applied directly to cells or tissue samples on microscope slides or coverslips. It is recommended to warm up at room temperature and mix by swirling the dropper vial before use. Store DAPI Ready Made Solution with antifade at -20 C protected from light.

    Caution

    Cell permeable fluorescent minor groove-binding probe for DNA. Binds to the minor groove of double-stranded DNA (preferentially to AT rich DNA), forming a stable complex which fluoresces approximately 20 times greater than DAPI alone.

    Footnote

    • Protects dyes from fading during imaging.
    • Ready-to-use liquid.
    • Easy to use Dropper vial application.
    • Stained sample are stable for longer time period.
    • Maintains fluorescence signal-little to no quenching.

    Immunogen

    DAPI, 4′,6-diamidino-2-phenylindole, is a blue fluorescent nucleic acid stain that fluoresces brightly upon selectively binding to the minor groove of double stranded DNA. Its selectivity for DNA and high cell permeability allows efficient staining of nuclei with little background from the cytoplasm. The excitation and emission wavelengths of DAPI-DNA complex are 360 nm and 460 nm, respectively. Most of the fluorescent dyes fade (photobleach) when exposed to excitation light. The amount of photobleaching depends on the composition of the molecule and the time of light exposure. DAPI Ready Made Solution with Antifade is a formulation that suppress photobleaching and prolong fluorescent signals.

    Physical properties

    Liquid - DAPI readymade with antifade solution is provided at 1μ

    Preparation Note

    g/mL concentration in glycerol-PBS based solution.

    SAFETY INFORMATION

    Signal Word

    H412

    P273 - P501

    RIDADRRIDADR

    WGK Germany

    WGK 2

    Flash Point (F)

    Not applicable

    Flash Point (C)

    Not applicable

    Target Organs

    Risk Statement

    Supplemental Hazard Statements

    DOCUMENTATION

    Certificate of Analysis View Sample COA
    Lot Number
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    Certificate of Origin
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