N5661
Sigma-Aldrich

NUCLEASE S1

Synonyms English:

Endonuclease S1

Nacres: NA.54
Mdl No: MFCD00131010
Grouped product items
SKU Pack Size Availability Price Quantity
N5661-50KU - Available to ship on March 28, 2024
IDR 5,563,000.00
Request a Bulk Order Check Availability

Properties

Properties
biological source Aspergillus sp. (A. oryzae)
concentration
concentricity 100000 
concentricity × camber units/mL
Description NUCLEASE S1
fragrance allergen solution
MDM Code N5661-50KU
Material Number N5661
Business Unit 760
Shipping Mode AIR
MRP Type PD
Local HS Code 35079000
Legacy SIGMA
Plant 6311
Sales Org 6304

Description

Application

Nuclease S1 from Aspergillus oryzae has been used in a study to assess a biochemical method for mapping mutational alterations in DNA. It has also been used in a study to investigate the DNA damage and repair in a γ

Biochem/physiol Actions

-irradiated rat brain tumor.

Caution

Nuclease S1 isolated from Aspergillus oryzae exhibits endo- and exolytic hydrolytic activity for the phosphodiester bonds of single-stranded DNA and RNA yielding 5’-phosphomononucleotide and 5’-phosphooligonucleotide end-products. It is used to digest non-annealed polynucleotide tails and hairpin loops in RNA and DNA duplexes and can be used to convert superhelical DNA to the linear form.

Immunogen

The Nuclease S1 enzyme from Aspergillus oryzae has the ability to degrade single-stranded oligonucleotides composed of either deoxynucleotides or ribonucleotides.

Physical properties

Solution containing 30 mM sodium acetate, 50 mM NaCl, 1 mM ZnCl2, 50% glycerol, 2 mg/ml protein

Warning

One unit will cause 1.0 microgram of single-stranded nucleic acid to become perchloric acid soluble per minute at pH 4.6 at 37°C.

SAFETY INFORMATION

Signal Word

RIDADRRIDADR

WGK Germany

WGK 2

Flash Point (F)

Not applicable

Flash Point (C)

Not applicable

Target Organs

Risk Statement

Supplemental Hazard Statements

DOCUMENTATION

Certificate of Analysis View Sample COA
Certificate of Origin

TECHNICAL SERVICE

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