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GENELUTE BACTERIAL GENOMIC DNA KIT, 70 P

GENELUTE BACTERIAL GENOMIC DNA KIT, 70 P

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Documents SDS COO/COA Specification Sheet

NA2110

Sigma-Aldrich

GENELUTE BACTERIAL GENOMIC DNA KIT, 70 P

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Nacres: Bacterial Genomic DNA Kits

Grouped product items
SKU	Pack Size	Availability	Price	Quantity
NA2110-1KT	-	Available to ship on October 21, 2023	IDR4,544,000.00		Add to Favorite List
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Documents SDS COO/COA Specification Sheet

Properties

Properties
Description	GENELUTE BACTERIAL GENOMIC DNA KIT, 70 P
MDM Code	NA2110-1KT
Material Number	NA2110
Business Unit	687
Shipping Mode	AIR
MRP Type	YP
Local HS Code	38229090
Legacy	SIGMA
Plant	6311
Sales Org	6304

Description

Application

The purified bacterial genomic DNA is ready for downstream applications such as:

restriction endonuclease digestions
PCR
Southern blots
cloning

Features and Benefits

Starting material: Up to 1.5 mL of culture
Expected yield: Up to 20 μg
Elution volume: 400 μl
Time required: 70 - 120 min
A₂₆₀/A₂₈₀ ratio: 1.6 - 1.9
No phenol, chloroform, or ethanol precipitation required

General description

The GenElute Bacterial Genomic DNA kit provides a simple and convenient way to isolate pure genomic DNA from gram-negative bacteria. For most gram-positive bacteria, the kit must be used in conjunction with the optional lysozyme (L4919) to effectively lyse the thick peptidoglycan cell walls. A Gram-Positive Lysis Solution is provided as a diluent for preparing the lysozyme stock solutions.

The kit combines the advantages of a silica-based system with a microspin format and eliminates the need for expensive resins, alcohol precipitation, and hazardous organic compounds such as phenol and chloroform.

Other Notes

For additional information, please see www.sigma-aldrich.com/genomicdna.

Principle

The bacteria are lysed in a chaotropic salt-containing solution to ensure the thorough denaturation of macromolecules. The addition of ethanol causes the DNA to bind when the lysate is spun through a silica membrane into a microcentrifuge tube. After washing to remove the contaminants, the DNA is eluted in 200 μL of a Tris-EDTA solution.

The expected yield of genomic DNA will vary depending on the cell density of the bacterial culture and the bacterial species and strain used. DNA purified with the GenElute kit has an A₂₆₀/A₂₈₀ ratio between 1.6 and 1.9 and can be up to 50 kb in length.

SAFETY INFORMATION

Pictograms

GHS02,GHS05,GHS07,GHS08,GHS09

Signal Word

Danger

Hazard Statements

H226 - H290 - H302 - H315 - H318 - H334 - H336 - H410

Precautionary Statements

P210 - P273 - P280 - P301 + P312 - P303 + P361 + P353 - P305 + P351 + P338

RIDADRRIDADR

WGK Germany

WGK 3

Flash Point (F)

77.0 °F - closed cup

Flash Point (C)

25 °C - closed cup

Target Organs

Central nervous system

Risk Statement

Supplemental Hazard Statements

DOCUMENTATION

Certificate of Analysis View Sample COA

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Certificate of Origin

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TECHNICAL SERVICE

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.