Proteinase K from Tritirachium album buffered aqueous glycerol solution, for molecular biology, >=800 units/mL
MDM Code
P4850-5ML
Material Number
P4850
Business Unit
687
Shipping Mode
AIR
MRP Type
PD
Local HS Code
35079000
Legacy
SIGMA
Plant
6311
Sales Org
6304
CAS Number
39450-01-6
Description
Application
Proteinase K from Tritirachium album has been used:
in bovine endometrial epithelial cells for herpes viral DNA extraction
for viral RNA extraction from nasal swabs
as a component of phase lock and direct PCR lysis buffer
Biochem/physiol Actions
Proteinase K is a stable and highly reactive serine protease. Evidence from crystal and molecular structure studies indicates the enzyme belongs to the subtilisin family with an active-site catalytic triad (Asp39-His69-Ser224). It is stable in a broad range of environments: pH, buffer salts, detergents (SDS), and temperature. In the presence of 0.1-0.5% SDS, proteinase K retains activity and will digest a variety of proteins and nucleases in DNA preparations without compromising the integrity of the isolated DNA.
Physical form
Solution in 40% glycerol (v/v) containing 10 mM Tris-HCl, pH 7.5, with 1 mM calcium acetate.
Unit Definition
One unit will hydrolyze urea-denatured hemoglobin to produce color equivalent to 1.0 μ
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