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    • Documents SDS COO/COA Specification Sheet
    P4850
    Sigma-Aldrich

    Proteinase K from Tritirachium album

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    Nacres: K from Tritirachium album
    CAS Number: 39450-01-6
    Grouped product items
    SKU Pack Size Availability Price Quantity
    P4850-5ML 5ML Available to ship on November 07, 2023
    IDR3,682,000.00
    P4850-1ML - Available to ship on June 29, 2024
    IDR1,858,000.00
    Request a Bulk Order Check Availability
    Documents SDS COO/COA Specification Sheet

    Properties

    Properties
    Description Proteinase K from Tritirachium album buffered aqueous glycerol solution, for molecular biology, >=800 units/mL
    MDM Code P4850-5ML
    Material Number P4850
    Business Unit 687
    Shipping Mode AIR
    MRP Type PD
    Local HS Code 35079000
    Legacy SIGMA
    Plant 6311
    Sales Org 6304
    CAS Number 39450-01-6

    Description

    Application

    Proteinase K from Tritirachium album has been used:
    • in bovine endometrial epithelial cells for herpes viral DNA extraction
    • for viral RNA extraction from nasal swabs
    • as a component of phase lock and direct PCR lysis buffer

    Biochem/physiol Actions

    Proteinase K is a stable and highly reactive serine protease. Evidence from crystal and molecular structure studies indicates the enzyme belongs to the subtilisin family with an active-site catalytic triad (Asp39-His69-Ser224). It is stable in a broad range of environments: pH, buffer salts, detergents (SDS), and temperature. In the presence of 0.1-0.5% SDS, proteinase K retains activity and will digest a variety of proteins and nucleases in DNA preparations without compromising the integrity of the isolated DNA.

    Physical form

    Solution in 40% glycerol (v/v) containing 10 mM Tris-HCl, pH 7.5, with 1 mM calcium acetate.

    Unit Definition

    One unit will hydrolyze urea-denatured hemoglobin to produce color equivalent to 1.0 μ

    Warning

    mole of tyrosine per min at pH 7.5 at 37 

    SAFETY INFORMATION

    GHS08

    Signal Word

    Danger

    H334

    P261 - P284 - P501

    RIDADRRIDADR

    WGK Germany

    WGK 1

    Flash Point (F)

    Not applicable

    Flash Point (C)

    Not applicable

    Target Organs

    Risk Statement

    Supplemental Hazard Statements

    DOCUMENTATION

    Certificate of Analysis View Sample COA
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    Certificate of Origin
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