[3:39 PM] Rifki Akbar
P4850
Sigma-Aldrich

Proteinase K from Tritirachium album

Nacres: K from Tritirachium album
CAS Number: 39450-01-6
Grouped product items
SKU Pack Size Availability Price Quantity
P4850-5ML 5ML Available to ship on May 02, 2024
IDR 3,682,000.00
P4850-1ML - Available to ship on December 23, 2024
IDR 1,858,000.00
Request a Bulk Order Check Availability

Properties

Properties
Description Proteinase K from Tritirachium album buffered aqueous glycerol solution, for molecular biology, >=800 units/mL
MDM Code P4850-5ML
Material Number P4850
Business Unit 687
Shipping Mode AIR
MRP Type PD
Local HS Code 35079000
Legacy SIGMA
Plant 6311
Sales Org 6304
CAS Number 39450-01-6

Description

Application

Proteinase K from Tritirachium album has been used:
  • in bovine endometrial epithelial cells for herpes viral DNA extraction
  • for viral RNA extraction from nasal swabs
  • as a component of phase lock and direct PCR lysis buffer

Biochem/physiol Actions

Proteinase K is a stable and highly reactive serine protease. Evidence from crystal and molecular structure studies indicates the enzyme belongs to the subtilisin family with an active-site catalytic triad (Asp39-His69-Ser224). It is stable in a broad range of environments: pH, buffer salts, detergents (SDS), and temperature. In the presence of 0.1-0.5% SDS, proteinase K retains activity and will digest a variety of proteins and nucleases in DNA preparations without compromising the integrity of the isolated DNA.

Physical form

Solution in 40% glycerol (v/v) containing 10 mM Tris-HCl, pH 7.5, with 1 mM calcium acetate.

Unit Definition

One unit will hydrolyze urea-denatured hemoglobin to produce color equivalent to 1.0 μ

Warning

mole of tyrosine per min at pH 7.5 at 37 

SAFETY INFORMATION

Signal Word

Danger

P261 - P284 - P501

RIDADRRIDADR

WGK Germany

WGK 1

Flash Point (F)

Not applicable

Flash Point (C)

Not applicable

Target Organs

Risk Statement

Supplemental Hazard Statements

DOCUMENTATION

Certificate of Analysis View Sample COA
Certificate of Origin

TECHNICAL SERVICE

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