Library preparation is the first step of next generation sequencing. Before DNA or RNA samples can be sequenced, nucleic acids must be isolated, fragmented, end-repaired, and covalently linked to adapters using ligation or tagmentation methods. The complexity of a well-prepared NGS library should fully and accurately reflect the complexity of the sample, but will also reflect any biases introduced during library preparation. A key goal in preparing a DNA or RNA library for next generation sequencing is to maximize complexity while reducing PCR or other amplification-introduced biases, as the quality of the resulting library can strongly impact NGS results.
We offer a portfolio of NGS library preparation tools designed to simplify workflows and facilitate the preparation of high quality DNA or RNA libraries that accurately represent sample complexity while reducing bias for whole genome sequencing (WGS), whole transcriptome analysis (WTA), total RNA sequencing, and miRNA and small RNA sequencing applications.
The microbiome refers to the collective genomes or genetic material of all microbes in a particular environment, called the microbiota. The human microbiome describes the collected microbiomes of the human body that reside primarily in the gut, and that vary considerably from one individual to another and among different anatomical sites. Factors that can influence the microbiome include diet, lifestyle, genetics, anatomical site, antibiotics and pathogens.
Explore our rich offering of microbiome products and services below.
MICROBIOME RESEARCH
In the emerging field of microbiome research, scientists are challenged to identify and characterize unknown microbes that can be difficult to isolate, culture, and study. Applications of human, animal, and environmental microbiome analyses have the potential to lead to the discovery of new therapeutic and natural products. Our scientists are creating new solutions to help you culture and/or identify microbes for characterization of microbial communities.
To enable the discovery of novel microbes, we offer:
Epigenetics describes changes that are stable, but potentially reversible alterations in gene expression, that occur without permanent changes in DNA sequence and can still be passed on from generation to generation. Epigenetically controlled genes are activated or repressed without any change in DNA. Three central epigenetic mechanisms that play an essential role in gene regulation have been extensively studied by researches, including DNA methylation, histone modification, and RNA regulation. Our combined comprehensive epigenetics portfolio offers high quality products to perform the techniques used to study all the three central epigenetic mechanisms.
Breakthroughs are driven not only by inquisition and keen insight into biological processes but also by the molecular tools currently available. Progressing your research often employs a multifaceted approach utilizing genomics, proteomics, and cellular analyses. In order to enable your next discovery, we exclusively offer the complete Roche portfolio of biochemical reagents, as well as a comprehensive range of their qPCR and nucleic acid purification products. Through the spirit of collaborative innovation, our partnership with Roche includes our combined rich legacies and drive for continued product quality excellence to truly offer a seamless experience and a world-class suite of advanced molecular tools. For additional information on the power of our unique collaboration with Roche, please visit our Roche distribution agreement FAQ page.
As pioneers and current leaders in the field of PCR, Roche nucleic acid purification, PCR and qPCR products provide you with assurance that your research is valid from the start. Additionally, our suite of reagents for DNA transfection, cell isolation and function analyses, and our protein protease and phosphatase inhibitors provide end-to-end solutions throughout your gene and protein expression workflows. We invite you to explore our combined offerings and discover how we can help you drive your next discovery.
GENOMICS RESEARCH – PCR, qPCR, RT-PCR, RT-qPCR, AND DIG-LABELING REAGENTS AND PROTOCOLS
An essential technique within virtually every molecular biology lab, polymerase chain reaction (PCR) is an easy and affordable method for amplifying specific fragments of DNA by several orders of magnitude. Our specialized kits have been optimized for a variety of PCR applications. Enabling room temperature PCR reaction setup with hot start PCR reagents, FastStart™ Taq DNA Polymerase is also compatible with automated setups.
Whether you need Kapa Biosystems PCR products for DNA extraction and amplification, or Kapa qPCR and qRT-PCR products utilizing SYBR® Green reagents, we have your PCR needs covered. The SYBR® Green-based qPCR reagent uses fluorescent dye that binds to double-stranded DNA molecules in order for researchers to quantify the amount of DNA that has been amplified. Probe-based qPCR reagents, such as FastStart™ Universal Probe Master (ROX), is suitable for use on all real-time PCR instruments that normalize with ROX. For RT-PCR/qRT-PCR applications, the Transcriptor One-Step RT-PCR Kit is designed for fast, sensitive, and specific end-point RT-PCR analysis of RNA. Additional nucleic acid labeling applications are readily met with the DIG-System for membrane hybridization and in situ hybridization needs.
PROTEOMICS ANALYSIS – PROTEIN STABILIZATION REAGENTS AND PROTOCOLS
It is essential to prevent the degradation or aggregation of the protein of interest as those factors will likely negatively impact the protein’s overall structure and functionality. This can be achieved through the addition of protease and phosphatase inhibitors to buffers and solutions. Widely cited, cOmplete™ and PhosSTOP™ protease and phosphatase inhibitors from Roche are easy-to-use, flexible tablet-based formulations which inhibit a wide range of proteases and phosphatases respectively. With the cOmplete™ Protease Inhibitor Cocktail, each of the water-soluble tablets contains a broad blend of metalloproteases, serine, and cysteine protease inhibitors to ensure the protection of proteins isolated from almost any tissue or cell. Additionally, the PhosSTOP™ Phosphatase Inhibitor Cocktail tablet provides a quick-dissolving, water-soluble formulation that contains a proprietary blend of acid and alkaline phosphatase inhibitors, serine/threonine phosphatase inhibitors and tyrosine protein phosphatase inhibitors.
CELL RESEARCH – CELL FUNCTION, CELL ISOLATION, AND TRANSFECTION REAGENTS AND PROTOCOLS
Involving the introduction of DNA or RNA into eukaryotic cells, transfection is a widely used technique for the study and modulation of gene expression. Created to afford high transfection efficiency, ease-of-use and reproducibility, X-tremeGENE™ Transfection Reagents provide increased viable cells and more physiologically relevant results for both transfection and siRNA reagents to knock-down gene expression. While many techniques are used to generate data from heterogeneous cell populations, it is often more informative to separate and analyze individual cellular sub-types. Collagenase and Liberase™ Research Grade enzymes are suitable for use with a broad range of cell and tissue types. Additionally, understanding cellular function also highly informative for evaluating cellular health or suggesting whether a potential drug candidate may be cytotoxic. The Roche biochemical reagents portfolio includes optimized ELISA kits for the measurement of cellular proliferation and cell death.
