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Library preparation is the first step of next generation sequencing. Before DNA or RNA samples can be sequenced, nucleic acids must be isolated, fragmented, end-repaired, and covalently linked to adapters using ligation or tagmentation methods. The complexity of a well-prepared NGS library should fully and accurately reflect the complexity of the sample, but will also reflect any biases introduced during library preparation. A key goal in preparing a DNA or RNA library for next generation sequencing is to maximize complexity while reducing PCR or other amplification-introduced biases, as the quality of the resulting library can strongly impact NGS results.
We offer a portfolio of NGS library preparation tools designed to simplify workflows and facilitate the preparation of high quality DNA or RNA libraries that accurately represent sample complexity while reducing bias for whole genome sequencing (WGS), whole transcriptome analysis (WTA), total RNA sequencing, and miRNA and small RNA sequencing applications.
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|Product Number||Product Description||Pricing|
|D7295||10 mM DEOXYNUCLEOTIDE MIX MOLECULAR BIO&||Expand|
|L1043||10X LYSIS AND FRAGMENTATION BUFFER||Expand|
|WTA2||COMPLETE WHOLE TRANSCRIPTOME AMPLIFICAT&||Expand|
|AMPD1||DEOXYRIBONUCLEASE I, KIT AMPLIFICATION &||Expand|
|PCR9604||GENELUTE 96 WELL PCR CLEAN-UP KIT||Expand|
|NA1020||GENELUTE PCR CLEAN-UP KIT||Expand|
|EC600||GenElute™-E DNA Cleanup Kit||Expand|