Description
Please refer to the attached
Protocolfor details.
Human tumor necrosis factor-alpha (TNF-α) ELISA Kit has been used in enzyme-linked immunosorbent assay (ELISA) to quantify TNF-α in supernates of human acute lung infection model (ALIM), human umbilical vein endothelial cells (HUVEC) and human microvascular endothelial cells (HMEC-1).
Tumor necrosis factor-α (TNF-α) is a key immunomodulator and proinflammatory cytokine. It is implicated in the immune pathogenesis of various autoimmune, inflammatory diseases and hepatitis B virus (HBV) infection. Mutation in the gene increases the risk of susceptibility to diffuse large B-cell lymphoma (DLBCL) and marginal zone lymphoma. In addition, polymorphism in the gene leads to obsessive-compulsive disorder (OCD) and osteoarthritis (OA).
This ELISA kit shows no cross-reactivity with any of the cytokines tested: Human ANG, CD23, Eotaxin, GCSF, GM-CSF, GRO-alpha, GRO-beta, GRO-gamma, I-309, IFN-gamma, IL-1 alpha, IL-1 beta, IL-3, IL-4, IL-5, IL-6, IL-7, IL-8, IL-10, IL-12 (p40), IL-12 (p70), IL-15, IL-16, IP-10, MCP-1, MCP-2, MCP-3, MCP-4, MCSF, MIG, MIP-1 alpha, MIP-1 beta, NAP-2, PDGF, PF-4
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