S3401
Sigma-Aldrich

Sample Buffer, Laemmli 2x Concentrate

Synonyms English:

SDS Sample Buffer

Nacres: NA.25
Grouped product items
SKU Pack Size Availability Price Quantity
S3401-10VL 10VL Available to ship on March 31, 2024
IDR 1,234,000.00
Request a Bulk Order Check Availability

Properties

Properties
Description Sample Buffer, Laemmli 2x Concentrate
MDM Code S3401-10VL
Material Number S3401
Business Unit 69E
Shipping Mode AIR
MRP Type PD
Local HS Code 38249999
Legacy SIGMA
Plant 6311
Sales Org 6304
CAS Number 0

Description

Application

Sample Buffer, Laemmli 2× concentrate has been used as a sample buffer for denaturing and loading of protein samples in SDS-PAGE.

Biochem/physiol Actions

Laemmli sample buffer is especially formulated for protein sample preparation to be used in the Laemmli SDS-PAGE system. A protein sample is mixed with the 2X sample buffer (1:1) and heated in boiling water for 2-5 min. The 2-mercaptoethanol reduces the intra and inter-molecular disulfide bonds. The SDS detergent denatures the proteins and subunits and gives each an overall negative charge so that each will separate based on size. The bromophenol blue serves as a dye front that runs ahead of the proteins and also serves to make it easier to see the sample during loading. The glycerol increases the density of the sample so that it will layer in the sample well.

Other Notes

Solution contains 4% SDS, 20% glycerol, 10% 2-mercaptoethanol, 0.004% bromphenol blue and 0.125 M Tris HCl, pH approx. 6.8.

Quantity

Recommended usage: add 1 volume gel loading solution to 1 volume sample and mix well.

SAFETY INFORMATION

GHS05,GHS06,GHS08

Signal Word

Danger

H302 - H311 - H315 - H317 - H318 - H361d - H373 - H412

P273 - P280 - P301 + P312 - P302 + P352 + P312 - P305 + P351 + P338 - P308 + P313

RIDADRRIDADR

WGK Germany

WGK 3

Flash Point (F)

Not applicable

Flash Point (C)

Not applicable

Target Organs

Risk Statement

Supplemental Hazard Statements

DOCUMENTATION

Certificate of Analysis View Sample COA
Certificate of Origin

TECHNICAL SERVICE

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