[3:39 PM] Rifki Akbar
SAB4200799
Sigma-Aldrich

ANTI-SHIGA TOXIN 1, B SUBUNIT (STXB)-FI&

Synonyms English:

Anti-SLT-1 B subunit; Anti-SLT-1b, Verocytotoxin 1 subunit B; Anti-Shiga-like toxin 1 subunit B; Anti-Verotoxin 1 subunit B; Anti-stxB

Nacres: NA.41
Grouped product items
SKU Pack Size Availability Price Quantity
SAB4200799-100UL - Available to ship on May 02, 2024
IDR 7,608,000.00
Request a Bulk Order Check Availability

Properties

Properties
antibody form purified from hybridoma cell culture
antibody product type primary antibodies
biological source mouse
clone 13C4 monoclonal
concentration ~1 
concentricity mg/mL
connections o.d. FITC conjugate
Description ANTI-SHIGA TOXIN 1, B SUBUNIT (STXB)-FI&
detection method Research area: Microbiome
format buffered aqueous solution
MDM Code SAB4200799-100UL
Material Number SAB4200799
Business Unit 696
Shipping Mode COU
MRP Type YP
Local HS Code 30021500
Legacy SIGMA
Plant 6311
Sales Org 6304

Description

Application

Anti-Shiga Toxin 1, B Subunit-FITC antibody, Mouse monoclonal may be used in flow cytometry.

Biochem/physiol Actions

The STX catalytic A subunit has RNA N-glycosidase activity that inhibits eukaryotic protein synthesis. The B subunits form a pentamer, which recognizes and binds to the functional cell-surface receptor globotriaosylceramide [Gb3, Galα (1-4)-Galβ (1-4)-Glcβ1-ceramide]. Shiga toxins are implicated in bloody diarrhea and hemorrhagic colitis in humans, which may lead to fatal systemic complications. Gb3 is overexpressed in membranes of numerous tumor cells, therefore, STxB binding to Gb3 receptors may be useful in cancer cell imaging.

Disclaimer

Unless otherwise stated in our catalog, our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

Monoclonal Anti-Shiga Toxin 1, B Subunit-FITC (mouse IgG1 isotype) is derived from the hybridoma 13C4 produced by the fusion of mouse myeloma cells and splenocytes from BALB/c mice. The Shiga toxins are a family of related protein toxins secreted by certain types of bacteria. Shiga toxin (Stx) is produced by Shigella dysenteriae; whereas, the Shiga-like toxins, Stx1 and Stx2, with a few known isoforms, are secreted by specific strains of Escherichia coli named Shiga-toxin-producing E. coli (STEC) such as E. coli O157:H7. Stx1 is identical to Stx and the Stx2 isoforms shares 60% similarity with STX. Howevere, the toxin structure is conserved. Shiga toxins consist of two polypeptides: A and B.

Immunogen

Purifed Shiga-like toxin from E. coli H30.

Other Notes

In order to obtain best results in different techniques and preparations we recommend determining optimal working concentration by titration test.

Physical form

Supplied as a solution in 0.01 M phosphate buffered saline pH 7.4 and 15 mM sodium azide as a preservative.

Specificity

Monoclonal Anti-Shiga Toxin 1, B Subunit -FITC recognizes the B subunit of Shiga holotoxin.

Storage and Stability

For continuous use, store at 2–8 °C protected from light for up to one month. For extended storage, freeze in working aliquots. Repeated freezing and thawing is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilution samples should be discarded if not used within 12 hours. Protect from prolonged exposure to light.

SAFETY INFORMATION

Signal Word

RIDADRRIDADR

WGK Germany

WGK 3

Flash Point (F)

Flash Point (C)

Target Organs

Risk Statement

Supplemental Hazard Statements

DOCUMENTATION

Certificate of Analysis View Sample COA
Certificate of Origin

TECHNICAL SERVICE

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.