[3:39 PM] Rifki Akbar
SAE0107
Sigma-Aldrich

3-METHYLADENINE READY MADE SOLUTION

Synonyms English:

3-MA

CAS Number: 5142-23-4
Grouped product items
SKU Pack Size Availability Price Quantity
SAE0107-10ML - Available to ship on April 25, 2024
IDR 4,864,000.00
Request a Bulk Order Check Availability

Properties

Properties
assay
assay range 98% (HPLC)
concentricity 7.5 
concentricity × camber mg/mL
Description 3-METHYLADENINE READY MADE SOLUTION
MDM Code SAE0107-10ML
Material Number SAE0107
Business Unit 710
Shipping Mode COU
MRP Type PD
Local HS Code 38249999
Legacy SIGMA
Plant 6311
Sales Org 6304
CAS Number 5142-23-4

Description

Application

3-Methyladenine (3-MA) is used to inhibit and study the mechanism of autophagy (lysosomal self-degradation) and apoptosis under various conditions. 3-MA inhibits autophagy by blocking autophagosome formation via the inhibition of type III Phosphatidylinositol 3-kinases (PI-3K). For use as an autophagy inhibitor, 3-MA is typically used at a concentration of 5mM.

General description

3-Methyladenine (3-MA)  is a methylated derivative of adenine that has been used as an In vivo marker for the methylation of DNA. 3-Methyladenine and 7-methylguanine are the two main N-methylpurines formed in DNA upon exposure to methylating agents. 3-MA is removed from damaged DNA via the DNA base excision repair pathway, which begins with the enzyme N-methylpurine DNA glycosylase. The dependence of 3-MA repair on nucleotide excision repair in mammalian cell lines has been studied.3-MA have been widely used as autophagy inhibitor based on its inhibitory effect on class I and class III PI3K activity, which is known to be essential for induction of autophagy. 3-MA blocks class I PI3K persistently, whereas its suppressive effect on class III PI3K is transient, understanding the dual role of 3-MA in autophagy thus suggests that caution should be exercised in the application of 3-MA in autophagy study.
Furthermore, 3-MA is also capable of making the reduction of lymphocyte number caused by chronic stress rebound, implying that inhibition of autophagy can rescue chronic stress-induced immunosuppression.
Autophagy has been reported to play an important role in the 3, 4-Methylenedioxymethamphetamine MDMA-induced neurotoxicity in the mixed neuronal-glial primary culture and it was found that 3-MA ameliorate MDMA-induced autophagy, SERT loss, HPA axis dysfunction, and depressive behaviors.

Preparation Note

3-Methyladenine ready made solution is provided at 50mM (7.5mg/mL) concentration in MES buffer.
It is recommended to store 3-methyladenine readymade solution at -20C.
In case percipitates are formed upon thaw, use sonication and heat to get the product back into solution.
It is recommended to avoid freeze thaw cycles.  
When stored properly 3-Methyladenine readymade solution should be stable for 2 years.

SAFETY INFORMATION

Signal Word

RIDADRRIDADR

WGK Germany

WGK 2

Flash Point (F)

Not applicable

Flash Point (C)

Not applicable

Target Organs

Risk Statement

Supplemental Hazard Statements

DOCUMENTATION

Certificate of Analysis View Sample COA
Certificate of Origin

TECHNICAL SERVICE

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.