T0440
Sigma-Aldrich

3,3'5,5',-TETRAMETHYLBENZIDINE (TMB) LIQ

Synonyms English:

TMB

Nacres: NA.83
Grouped product items
SKU Pack Size Availability Price Quantity
T0440-100ML - Available to ship on April 02, 2024
IDR 3,212,000.00
T0440-1L - Available to ship on April 02, 2024
IDR 20,392,000.00
T0440-5X1L - Available to ship on April 02, 2024
IDR 47,838,000.00
Request a Bulk Order Check Availability

Properties

Properties
Description 3,3'5,5',-TETRAMETHYLBENZIDINE (TMB) LIQ
form liquid
MDM Code T0440-100ML
Material Number T0440
Business Unit 760
Shipping Mode AIR
MRP Type YP
Local HS Code 29215900
Legacy SIGMA
Plant 6311
Sales Org 6304

Description

Application

TMB substrate is used in ELISA procedures, which utilize horseradish peroxidase conjugates. TMB substrate develops a soluble blue reaction product that can be read at 370 or 655 nm at 370 or 655 nm. The reaction is stopped with acid, forming a yellow reaction product which enables accurate intensity measurement at 450 nm.

How to use our TMB substrate:
  • Our TMB substrate is supplied as a ready-to-use peroxidase substrate containing TMB in a mildly acidic buffer. Prior to reaction with peroxidase, the substrate should be a colorless to light bluish-green solution. The substrate system develops a blue reaction product when reacted with peroxidase.
  • Upon reaching your assay endpoint with addition of acid, measure the TMB substrate intensity at 450 nm.
  • Our TMB Substrate is recommended for ELISA (microwell) procedures, and has been used in various assays, as a substrate for horseradish peroxidase antibody conjugate for detecting hendra virus glycoprotein, tetracosactide, sIgA and neutrophil extracellular traps (NETs).
  • 3,3′,5,5′-Tetramethylbenzidine (TMB) Liquid Substrate System is not recommended for membrane applications. Since TMB substrate produces a soluble reaction product, it is not recommended for histochemistry or blotting.
Note:TMB substrate is light-sensitive and should be protected from direct sunlight or UV sources.

General description

TMB substrate is a chromogenic substrate for horseradish peroxidase - HRP.

SAFETY INFORMATION

Signal Word

RIDADRRIDADR

WGK Germany

WGK 3

Flash Point (F)

Not applicable

Flash Point (C)

Not applicable

Target Organs

Risk Statement

Supplemental Hazard Statements

DOCUMENTATION

Certificate of Analysis View Sample COA
Certificate of Origin

TECHNICAL SERVICE

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