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T4904
Supelco

Tris-Glycine Buffer 10x Concentrate

Nacres: NA.25
Grouped product items
SKU Pack Size Availability Price Quantity
T4904-1L 1LT Available to ship on April 26, 2024
IDR 2,847,000.00
Request a Bulk Order Check Availability

Properties

Properties
Description Tris-Glycine Buffer 10x Concentrate
foreign activity Protease none detected
MDM Code T4904-1L
Material Number T4904
Business Unit 69E
Shipping Mode AIR
MRP Type PD
Local HS Code 38229090
Legacy SIGMA
Plant 6311
Sales Org 6304
CAS Number 0

Description

Application

Tris-Glycine buffer 10× concentrate has been used as a transfer buffer for Western blotting.

Biochem/physiol Actions

For Western blotting and gel electrophoresis. Tris-glycine buffer is used to make a Tris-glycine-methanol transfer buffer, which is the most common protein transfer buffer for wet blot transfers. The methanol prevents the gel from swelling during the transfer and enhances the protein binding to nitrocellulose.The 10× Tris-glycine buffer is diluted to 1× with methanol and water to make a solution containing 25 mM Tris, 192 mM glycine, and 20% methanol. A sufficient amount of transfer buffer should be made to cover the electrode wires in the wet blot transfer unit and to soak the gel, membrane and blotting paper.

Other Notes

0.25 M Tris, 1.92 M glycine, pH approx. 8.3
Contains no methanol.

SAFETY INFORMATION

Signal Word

RIDADRRIDADR

WGK Germany

Flash Point (F)

Flash Point (C)

Target Organs

Risk Statement

Supplemental Hazard Statements

DOCUMENTATION

Certificate of Analysis View Sample COA
Certificate of Origin

TECHNICAL SERVICE

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